Introduction to single-stranded DNA (ssDNA)
Single-stranded DNA (ssDNA) plays a crucial role in numerous biological processes, including DNA replication and repair. In cells, ssDNA typically occurs transiently when genomic DNA double helices separate into their two constituent strands. Furthermore, ssDNA serves as the genetic material for various virus families, such as adeno-associated viruses (AAV), which are extensively used as vectors in gene therapy. Beyond its biological significance, ssDNA is emerging as a valuable molecule for innovative applications in fields like DNA nanotechnology and gene editing.
ssDNA vs. dsDNA: key differences
Double-stranded DNA (dsDNA) consists of two complementary strands forming a helical structure, held together by hydrogen bonds. In contrast, single-stranded DNA (ssDNA) comprises only one strand, giving it greater flexibility than dsDNA. This flexibility makes ssDNA particularly valuable for nuanced applications such as gene editing and DNA nanotechnology, positioning it as an ideal solution for a wide range of scientific and medical purposes.
Why gxstrands ssDNA?
Long, scalable and versatile
Single-stranded DNA (ssDNA) is commonly produced in vitro through chemical synthesis or enzymatic processes. At gxstrands, we employ a fermentation-based approach that allows for the scalable production of ssDNA sequences, ranging from micrograms to tens of grams. We offer ssDNA with lengths of up to 10,000 nucleotides, tailored to meet the specific needs of our customers' research and industrial applications.
Quality & service features
To ensure the highest quality, our standards include nanopore sequencing and gel electrophoresis, guaranteeing that our products meet the stringent requirements of your applications. Our extra purified, endotoxin-free single-stranded DNA is ideal for in vivo research and cell culture experiments.
Delivery and storage of ssDNA
Turnaround time of gxstrands ssDNA
The average delivery time for ssDNA sequences is 6 weeks, though it may vary depending on the complexity and length of the requested sequence. After the successful completion of plasmid production, we will provide an updated timeline. For precise information regarding your specific delivery timeline, please contact us at info@gxstrands.com.
Delivery options for ssDNA
Usually, your single-stranded DNA is delivered freeze-dried at room temperature. However, other delivery options, such as in a specific buffer or at a specific concentration, are available upon request. At gxstrands, we ensure your ssDNA is delivered according to your specific requirements.
Storage and handling guidelines for ssDNA
Proper storage and handling of ssDNA are crucial for preserving its functionality and integrity. The stability of ssDNA depends on the buffer and storage temperature. For long-term preservation, we recommend storing ssDNA at -20 °C, and for short-term use, at 4 °C. Upon receiving ssDNA, resuspend it in nuclease-free water or TE-buffer to maintain stability and prevent degradation. For optimal results, please refer to our resuspension protocol in the FAQs.
Your partner for scalable ssDNA solutions
gxstrands is not just a supplier of ssDNA, we are your reliable partner for scaling up scientific breakthroughs and innovation, always ensuring high quality and accountability. Contact us via info@gxstrands.com to discuss how our ssDNA solutions can support your next project.
